摘要
目的 探讨缺血对移植心脏远期冠状血管的形态学影响。方法 雄性Wistar大鼠为供者和受者 ,建立大鼠腹部异位心脏移植模型。实验分为 4组 ,即假手术对照组、立即移植组、供心缺血 3h移植组和缺血 6h移植组。每组术后均用环孢素A(CsA)灌胃 2 0d ,抗排斥反应。 2 0d时 ,取出供心 ,观察心脏冠状血管病理改变及超微结构变化。结果 假手术对照组心脏和立即移植组的供心冠状血管内皮超微结构均无明显损害 ;随着供心缺血时间的延长 ,移植后冠脉小血管内皮细胞肿胀 ,冠状动脉的内皮细胞增生 ,内膜增厚 ;缺血 6h移植组供心冠状血管内膜超微结构改变非常明显。结论 供心缺血可导致移植后冠状血管内皮损伤 ,是心脏移植术后冠状血管病理改变的重要因素。
Objective To study the relationship between Cardiac allograft vasculopathy (CAV) and donor heart ischemia time. Methods The rat model of retroperitioneal heterotopic heart transplantation was used. Seventy Wistar rats were divided into 4 groups: group 1 receiving CsA after sham operation; group 2 receiving transplantation directly; group 3 having 3 h-ischemia before transplantation; group 4 having 6?h ischemia before transplantation. After transplantation, all rats in each group were subjected to the gastric lavage of CsA for 20?days. At 20?days after transplantation, the donor heart was removed for observation of changes in pathological lesions and ultrastructues of coronary artery. Results With ischemia time being prolonged, cardiac capillary endothelial cell swelling and coronary intimal proliferation were observed which were similar to the CAV histopathological changes. Conclusion The prolongation of ischemia time can lead to intimal proliferation and hyperplasia which can promote CAV development. So ischemia time is a vital reason for the CAV.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2003年第6期329-331,共3页
Chinese Journal of Organ Transplantation
基金
国家自然科学基金资助项目 ( 39870 711)
霍英东教育基金会优秀青年教师基金资助项目 ( 710 38)
