HIV/AIDS患者CD28在外周血CD4^+、CD8^+ T细胞上的表达变化

Change of expression of CD28 on peripheral CD4^+,CD8^+ T lymphocytes in HIV/AIDS patients

目的 研究国内HIV AIDS患者CD2 8在外周血CD4 + 、CD8+ T淋巴细胞上表达的变化 ,并探讨这些变化的临床意义。方法 用流式细胞仪检测 5 1例正常对照、14例HIV感染者和 36例AIDS患者的外周血CD4 + 、CD8+ T淋巴细胞表面的CD2 8分子的表达 ,用bDNA法检测 11例HIV感染者和 18例AIDS患者的血浆病毒载量。结果 CD4 + CD2 8+ T细胞的绝对计数与百分比、CD8+ CD2 8+T细胞的百分比均显示为正常对照组 >HIV感染组 >AIDS组 ;而CD8+ CD2 8+ T细胞的绝对计数显示HIV感染组和对照组显著大于AIDS组 ,HIV感染组与对照组间差异无显著性。CD4 + 、CD2 8+ CD4 + T淋巴细胞计数与血浆病毒载量显著负相关。结论 HIV AIDS患者外周血CD2 8在CD4 + 、CD8+ T淋巴细胞上表达随着病情进展而降低 ,反映了细胞免疫功能随着疾病进展损害逐渐加重 ,是判断病情进展的指标。

Objective This study was designed to investigate the change of expression of CD28 on CD4 +, CD8 + T lymphocytes in HIV/AIDS patients and to explore the clinical significance of the change. Methods Expression of CD28 on peripheral blood CD4 + and CD8 + T lymphocytes in 51 health blood donors, 14 asymptomatic HIV infected individuals and 36 AIDS patients was analysed by flow cytometry. The plasma viral load of 11 asymptomatic HIV infected individuals and 18 AIDS patients was determined by bDNA. Results Percentage of CD4 +CD28 + and CD8 +CD28 + cells, CD4 +CD28 + cell count showed a picture as AIDS group < HIV group < normal control; the count of CD8 +CD28 + cells in HIV group and controls was higher than that in AIDS group. There was negative correlation between CD4 +, CD4 +CD28 +count and plasma viral load. Conclusion The expression of CD28 on CD4 +, CD8 + T lymphocytes in HIV/AIDS patients decreased along with disease progress reflecting gradual impairment of cell-mediated immunity and poor prognosis.