非选择性ATP受体拮抗剂对坐骨神经再生轴突诱向作用影响的实验研究

The effect on sciatic nerve regeneration of non-selective antagonist of ATP receptors

目的 探讨苏拉明对细胞外ATP的轴突化学性诱向作用的影响。 方法 选用SD雌性大鼠 10只 ,制作成鼠坐骨神经缺损模型 ,将“Y”形硅胶管单臂一侧与坐骨神经近断端行套入缝合。“Y”形硅胶管的两管臂远端用无损伤线交叉或平行缝合封闭 ,以区别实验侧和对照侧。与左侧坐骨神经套入缝合的硅胶管两壁内分别注入 1mmol/LATP、生理盐水 10 μl和 1mmol/LATP加 0 2mg/ml苏拉明 10μl;与右侧坐骨神经套入缝合的硅胶管硅胶管两壁内分别注入生理盐水 10 μl和 0 2mg/ml苏拉明 10μl。术后 8周取标本进行肉眼、光镜、电镜及图像分析系统观察和检测再生神经的形态、有髓纤维的数目、形态、髓鞘厚度等。 结果 含ATP组再生神经干内有髓纤维的数目 (2 2 46± 2 2 2 )明显多于含生理盐水组 (10 61± 161) ,后者又明显多于含苏拉明组 (2 66± 84)。ATP受体非特异性拮抗剂苏拉明能完全阻断细胞外ATP对外周神经再生轴突的诱向作用。单纯的苏拉明对再生轴突也具有非常明显的抑制作用。

Objective To prove the extracellular ATP attracts the axonal regeneration after peripheral nerve injury via the ATP receptor Methods The sciatic nerve defects had been made in 10 female Sprague Danley rats and repaired by'Y' form silicon tube The single arm of the'Y' form tube were sutured with the proxma the sciatic nerve and the other two end of silicon tube sutured by paralled or cross method so as to differentiate the experimental side and control side, 1mmol/L 10 μl ATP and 1mmol/L 10 μm ATP+ 0 2mg/ml Suramin were injected differently into two arms of left side and 10 μl physiological saling and 10 μl 0 2mg/ml Suramin injected differently into two arms of right dide The silicon tube were removed after eight weeks and examine the form and number of myelinated tibers as well as the quanlity of the myelinated sheath Results In the left sides regeneration axon were tuned into the silicon tubes containing the ATP and there were not axon regeneration in the silicon tube contained the ATP and there were not axon regeneration in the silicon tube contained ATP+ Suramin In the right sides the regeneration axon were found only three tubes contained suramin and seven tubes contained physiological saline Conclusions The results demonstrated that the suramin inhibited the axon reduction of the extracellular ATP completely