透明质酸钠预防骨关节炎Ⅱ型胶原抗体水平的动态变化

Dynamic variety of humoral immunity in the course of preventing rabbit osteoarthritis by injection of sodium hyaluronate in knee

目的 研究关节内注射透明质酸钠 (海尔根 )预防膝骨关节炎 (OA)过程中Ⅱ型胶原抗体水平及其动态变化。方法 取 12只成年新西兰兔制备Hulth膝骨关节炎动物模型 ,随机等分为 2组。Ⅰ组术后当时及每 2周关节内注射 1ml 1%透明质酸钠 (Hyalgan)至 12周 ;Ⅱ组注射 1ml生理盐水为对照。各组术前及术后每隔 2周收集血清 ,建立间接法ELISA ,检测Ⅱ型胶原抗体水平。结果 Ⅰ组术后第 6、10、12周Ⅱ型胶原抗体水平与术前比较具有显著性差异 (P <0 .0 5 ) ;Ⅱ组Ⅱ型胶原抗体水平第 10周显著高于术前 (P <0 .0 5 ) ;Ⅰ组与Ⅱ组同期比较Ⅱ型胶原抗体水平均无显著差异。结论 OA使机体产生自身免疫 ,引起自身Ⅱ型胶原抗体水平增加。透明质酸钠能促进体液免疫应答 ,导致抗体水平进一步升高。提示 ,注射透明质酸钠治疗膝骨关节炎时 。

Objective Study the humoral immune status and its dynamic variety in the course of preventing osteoarthritis(OA) of knee by injection of sodium hyaluronate (SH;Hyalgan) in joint.?Methods 12 New Zealand Rabbits had been prepared for animal model of OA in knee,and divided randomly into two groups. Experimental groups were injected SH (1ml,1%) in joints after operation immediately and every two weeks up to 12 weeks. Physiological saline (1ml) was injected into the knee joints of the controls. Collect serum every two weeks before or after surgery operation in two groups. The antibodies of type Ⅱ collagen in serum was detected with indirect ELISA.?Results Comparing the times before and after operation,the antibody levels of type Ⅱ collagen in the 6th,10th,12th weeks were significantly higher than that found before operation(P<0.05) in experimental groups;In controls,the concentrations of collagen Ⅱ antibody in 10th weeks were higher than before operation(P<0.05). Whereas comparing the two groups,there were no remarkable difference at the same periods.?Conclusion OA of knee could cause autoimmunity to collagen in the body,increase the levels of serum anti auto type Ⅱ collagen antibody. Injection of sodium hyaluronate could enhance the humoral immune causing higher levels of antibody. It's suggested that we should take its effect of adjustment of immunity into account during collagen Ⅱ injection for preventing OA.