短波紫外线照射对创伤局部碱性成纤维细胞生长因子表达的影响

Effect of ultraviolet C irradiation on expression of basic fib roblast growth factor of wound in rats

目的 研究不同剂量短波紫外线 (ultravioletC ,UVC)照射对伤口肉芽组织中碱性成纤维细胞生长因子 (basicfibroblastgrowthfactor ,bFGF)表达的影响。方法 将每只实验大鼠背部制作 3个皮肤全层伤口 ,分别采用不同剂量 (15mJ/cm2 或 60mJ/cm2 )UVC照射大鼠背部伤口 ,持续 3d后 ,分别于第 7天、第14天及第 2 1天应用免疫组化法及原位杂交法观察伤口肉芽组织中bFGF的表达情况。结果 致伤后 7d ,60mJ/cm2 照射组及 15mJ/cm2 照射组bFGF表达均高于对照组 (P均 <0 .0 1) ,且以 60mJ/cm2 照射组增高的更为显著 ;致伤后 14d ,60mJ/cm2 照射组bFGF表达显著降低 ,低于 15mJ/cm2 照射组及对照组 (P均 <0 .0 5)。结论 在伤口愈合早期 ,采用不同剂量 (15mJ/cm2 或 60mJ/cm2 )UVC照射能明显促进伤口肉芽组织内bFGF的表达 ,其中剂量为 60mJ/cm2 的UVC照射其作用短暂而迅速 ,而剂量为 15mJ/cm2 的UVC照射其作用缓慢而持久。

Objective To investigate the influence o f ultraviolet C (UVC) irradiation of different doses on expression of basic fibrob last growth factor (bFGF) in granulation tissues of wound in rats. MethodsThirty Wistar rats were recruited and three wounds wer e made in each rat. The UVC irradiation of different doses (60mJ/cm2 and 15mJ/ cm2) were performed daily in two of the three wounds in each rat, respectively , and the UVC irradiation lasted for three days. The expression of bFGF both at the mRNA level and the protein level were observed by the methods of in situ hyb ridization and immunohistochemistry at 7 days, 14 days, 21 days after wound was made, respectively. ResultsAt the 7 days after woun d was made, the expression of bFGF in irradiation groups were higher than that i n the control group ( P <0.05) at both the mRNA and the protein level, especia lly the increment in the 60mJ/cm2 group was more significant. While at 14 day after the wound establishment, the expression of bFGF in the 60mJ/cm2 group de creased rapidly and the concentration of bFGF were lower than that in the other two groups ( P <0.05). At the same time, the expression of bFGF in the 15mJ/cm 2 group was higher than that in the 60mJ/cm2 group. Conclusi onIn the initial stage of wound healing, the treatment of UVC irrad iation promoted the expression of bFGF and the effect of 60mJ/cm2 irradiation was fast and transit, the effect of the 15mJ/cm2 irradiation was durative and slow. The increment of bFGF promoted by UVC irradiation might be the one of the mechanisms of UVC irradiation healing wound.