大鼠穴位针刺预处理抗脑缺血再灌注损伤作用探讨

Influence of Electroacupuncture Preconditioning on Surviving Neuron Density of Cerebral Cortex in Rats with Temporary Cerebral Ischemia-reperfusion

目的 :探讨穴位针刺预处理抗脑缺血再灌注损伤的作用。方法 :先进行“肾俞”、“百会”穴针刺预处理 ,再采用颈动脉引流法脑缺血再灌注造模 ,石蜡包埋切片 ,HE染色 ,观察脑片缺血性病理变化 ,进行顶皮质Ⅰ区Ⅴ层幸存神经元记数。结果 :在不同再灌时间段 ,E0 .5hr组幸存神经密度显著高于D组、E1 .5hr组和E3hr组 (P <0 0 5) ,E3hr组与D组无差异 (P >0 0 5)。结论 :穴位针刺预处理具有抗脑缺血再灌注损伤作用 ,该作用以穴位针刺预处理后 0

Objective:To observe the effect of electroacupuncture preconditioning (EAP) on neurons of the cerebral cortex in cerebral ischemia-reperfusion (IR) rats. Methods: 95 Wistar rats were randomly divided into normal control group (group A, n=5), sham-ischemia control group (group B, n=15), EAP control group (group C, n=15), cerebral IR control group (group D, n=15), EAP and cerebral IR group (group E, n=45). E group was further evenly divided into 0.5 hr, 1.5 hr and 3 hr subgroups (i.e. IR was conducted 0.5, 1.5 and 3 hours after EA). Temporary cerebral IR injury model was established by using common carotid artery blood drainage method. 'Shenshu' (BL 23) and 'Baihui' (GV 20) were punctured and stimulated electrically with an EA therapeutic apparatus (8～80 Hz, dense-sparse waves, 3 V, 4×10 min). After treatment, these rats of group B, C, D and 3 subgroups of group E survived for 1, 3 and 7 days (i.e. IR-1d, IR-3d and IR-7d) respectively, and were then killed for taking out the brain tissue which was to be cut into sections after perfusion fixation, with the tissue sections being stained with HE method. Then the surviving neurons were counted under microscope. Results: There were no significant differences among group A, B and C at IR-1d and between group B and C at IR-3d and IR-7d in the density of the cerebral surviving neurons, indicating that sham-operation and EAP had no apparent influence on cerebral neurons. Compared with group A, the density values of neurons of group D at IR-1d, IR-3d and IR-7d all reduced significantly (P<0.05). Comparison among E 0.5 hr, E 1.5 hr and E 3 hr subgroups of group D,displayed that the density values of E 0.5 hr subgroup at different IR time-courses and E 1.5 hr subgroup at IR-3d and IR-7d all evidently higher than those of group D(P<0.05), suggesting that EAP has an apparent anti-IR injury action. But there were no significant differences among E 1.5 hr and E 3 hr and D groups at the 3 time-courses (P>0.05).Conclusion: EAP can resist the injury of neurons of the cerebral cortex induced by temporary cerebral ischemia-reperfusion, which is better at 30 min after EAP than at 90 min and 180 min.