电针对局脑缺血大鼠再灌流后四种氨基酸含量的影响

Effects of Electroacupuncture on Levels of Glutamate, Aspartate, γ-Aminobuyric Acid and Glycine in Rat Brain after Cerebral Ischemia-reperfusion

目的 :观察电针对脑缺血大鼠谷氨酸、门冬氨酸、甘氨酸及氨基丁酸含量的影响。方法 :利用大鼠大脑中动脉阻塞再灌流模型 ,采用高效液相 色谱法测定四种氨基酸含量。结果 :大鼠局脑缺血 3 0min ,再灌流 4hr后 ,Glu、ASP含量明显升高 (P <0 0 1 ,P <0 0 5) ,GABA和Gly含量无明显变化 ;而经电针治疗组Glu、ASP含量显著下降 (P <0 0 1 ) ,GABA含量升高 (P <0 0 5) ,Gly含量无明显变化。结论 :电针可抑制兴奋性氨基酸的过量堆积 。

Objective: To study the effects of electroacupuncture (EA) on glutamate(Glu), aspartate(Asp), Glycine (Gly) and γ-Aminobuyric acid(GABA) in the brain tissue in rats with cerebral ischemia-reperfusion (CIR).Methods: Twenty-four Wistar rats were randomly and evenly divided into control group, CIR group and CIR+EA group. CIR model was established by occlusion of the middle cerebral artery for 30 min and removing the occlusion for 4 hours. 'Suliao' (GV 25) and 'Baihui' (GV 20) were punctured and stimulated with an EA therapeutic apparatus (dense-sparse waves, 5/ 45 Hz, 0.3 mA) for 30 min. High pressure liquid chromatography (HPLC)-fluorescence assay was used to detect contents of Glu, Asp, Gly and GABA in rat brain tissue before and after EA. Results: Four and half hours after CIR, Glu and Asp contents of the brain tissue increased significantly(P< 0.01, P<0.05),Gly and GABA levels had no obvious changes. After EA treatment, cerebral Glu and Asp levels reduced considerably (P<0.01), GABA level increased strikingly (P<0.05), and Gly content had no evident change. Conclusion: EA can reduce excessive accumulation of excitatory amino acids (Glu and Asp) in the brain that may be related to EA-induced increase of GABA. The regulative action of EA on excitatory and inhibitory amino acid contents in the brain may contribute to EA's neuroprotective effect on ischemic cerebral tissue.