不同时间窗电针对急性局灶性脑缺血模型大鼠脑组织活性钙调素含量影响的研究

Influence of Electroacupuncure at Different Therapeutic Time Windows on Calmodulin Content of the Brain Tissue in Focal Cerebral Ischemic Injury Rats

目的 :研究不同时间窗电针对实验性脑缺血疗效的影响。方法 :采用光化学法诱导局灶性脑缺血模型 ,于造模成功后 0 5hr、1hr、3hr、6hr、1 2hr五个不同的时间窗开始电针 ,比较治疗 6天后急性局灶性脑缺血模型大鼠脑组织活性钙调素 (CaM)含量的变化。结果 :大鼠脑缺血后 ,模型组CaM的含量明显升高 ,与空白对照组和假手术组相比有显著性差异 (P <0 0 5) ;电针治疗组的CaM含量与空白对照组相比无统计学差异 (P >0 0 5) ,但超早期不同的时间窗电针组间差异不显著 (P >0 0 5)。结论 :针刺疗法超早期介入在大鼠局灶性脑缺血中发挥肯定的脑保护作用 ,但从造模后 0

Objective:To observe the influence of electroacupuncure (EA) at different therapeutic time windows on calmodulin(CaM) level in focal cerebral ischemic injury rats. Methods: Seventy-three Wistar rats were randomized into normal control (n=10), sham-operation (n=8), model (n=9), EA-0.5 hr (n=10), EA-1 hr (n=10), EA-3 hr(n=8), EA-6 hr (n=9) and EA-12 hr (n=9) groups. The rat cerebral ischemia model was established by middle cerebral artery occlusion (MCAO) with photochemical method to induce focal cerebral ischemia injury. EA (20 Hz, 1 min/time) was delivered to 'Shuigou'(GV 26) through acupuncture needles 0.5 hr, 1 hr, 3 hr, 6 hr and 12 hr respectively following MCAO, once daily, continuously for 6 days. The active CaM content of the ischemic cerebral tissues was measured by using phosphodiesterase method. Results: In model group, CaM content of the ischemic cerebral region elevated distinctly in comparison with normal control group and sham-operation group(P<0.05). Following administration of EA 0.5 hr, 1 hr, 3 hr, 6 hr and 12 hr after MCAO, CaM contents of these 5 EA groups were all lowered evidently compared with that of model group(P<0.05). There were no evident differences between each different therapeutic time window groups and normal control group in CaM content (P>0.05), suggesting that acupuncture treatment given at these 5 time intervals can effectively reduce cerebral ischemia induced elevation of active CaM level of the rat brain tissue. Conclusion: When acupuncture treatment was administered in the early period of acute cerebral ischemia, the elevated active CaM level in the brain tissue can be corrected effectively.There is no obvious correlation between different therapeutic time windows and the effect of acupuncture.