链球菌G蛋白用于组织化学染色的实验研究

STUDIES ON THE APPLICATION OF STREPTOCOCCAL PROTEIN G IN HISTOCHEMICAL STAINING

用理化方法制得链球菌G蛋白(SPG)-辣根过氧化物酶及SPG-胶体金探针,并将其用于常规病理组织切片、电镜包埋后、包埋前及冰冻超薄切片的抗原定位。组织化学染色显示,SPG定位准确、标记密度高,背景清晰,显著优于经典的葡萄球菌A蛋白(SPA)。研究结果表明,SPG的一个最重要的优点就是在免疫组化染色条件下仍有着比SPA更稳定、更可靠、更强的IgG亲合力,尤其适用于单克隆抗体,可获得更为理想的标记结果,因而是一种能用于多种不同条件组织化学染色的新型、高效免疫学检测剂。

Horseradish peroxidase (HRP) and colloidal gold (Au) were physicochemically linked to streptococcal protein G (SPG)to produce SPG-HRP and SPG-Au probes. Subsequently, they were introduced to the routine pathological sections, EM post embedding, pre-embedding and ultrathineryosectioning techniques. The histoehemical staining revealed highly dense, precise labellings by the probes with a clear background, markedly superior to staining with classical staphylococcal protein A (SPA). The results demonstrated that one of the most important advantages of SPG was its capa bility to retain a stronger, stabler and more reliable IgG-binding affinity than that of SPA under the histchemical staining conditions. Furthermore, SPG was especially suitable for monoclonal antibodies. SPG could be used as a novel, highly effective immunological detecting agent for histochemical staining under various conditions.