子宫内膜异位症、流产、不孕患者抗子宫内膜抗体的研究

THE STUDY OF ANTIENDOMETRIAL ANTIBODIES IN PATIENTS WITH ENDOMETRIOSIS, HABITUAL ABORTION OR INFERTILITY

将子宫内膜用胶原酶消化后依序过250μm,40μm筛网,获内膜腺体。匀浆后上清流穿抗人血浆亲和层析柱,得纯化人子宫内膜抗原。用此抗原建立了检测抗子宫内膜抗体的免疫斑点试验。子宫内膜异位症(EMT)、流产、不孕患者阳性率分别为69.6％(16/23)、41.5％(17/41)和39.7％(23/58),均显著高于对照组(1/34、2.9％)(p<0.01)。在三组患者中,又以EMT患者最高(p<0.05)。

By means of collagenase(Type I) digestion,sequential sieving and affinity chroma tography, the human endometrial antigen was purified. A method of immunospot assay for determination of antiendometrial antibodies (EMAb) was developed. The results of clinical study showed that the positive rates of EMAb in patients with endometriosis (EMT), habitual abortion and infertility were 69.6%(16/23),41.5%(17/41) and 39.7% (23/58) respectively, all being significantly higher than that in control group (1/34, 2.9%)(p<0.001). within these groups, the highest positive rate occurred in the EMT patients. (p<0.05).