肺纤维化患者支气管肺泡洗出液细胞基因表达的研究

GENE EXPRESSIONS IN BRONCHOALVEOLAR LAVAGE CELLS FROM THE PATIENTS WITH LUNG FIBROSIS

本文以逆转录-DNA聚合酶链反应和U_(937)细胞cDNA文库的分离杂交方法研究了肺纤维化患者支气管肺泡洗出液(BAL)细胞的基因表达情况。结果表明,在大部分标本可检出白细胞介素-1β和肿瘤坏死因子αmRNA,在部分标本中可检出转化生长因子β和胰岛素样生长因子-I mRNA;肺纤维化患者的BAL细胞可表达出一些正常志愿者BAL细胞未表达的mRNA,而这些mRNA的拷贝存在于PMA刺激的U_(937)细胞cDNA文库中。

We have studied the gene expressions of bronchoalveoiar lavage (BAL) cells from the patients with lung fibrosis by a reverse transcription DNA polymerase chain reaction technique and by a differential screening of PMA stimulated U937 cell cDNA library. The results indicated that interleukin 1β mBNA and tumour necrosis factor α mRNA were detected in a great part of the samples. Transforming growth factorβ mRNA and insulin like growth factor I mRNA were detected in some of the samples. BAL cells from the patients with lung fibrosis expressed more mRNA species, whose copies existed in PMA stimulated U937 cell cDNA library, than those from normal volunteers.