摘要
目的 观察 72h睡眠剥夺大鼠的氧化应激状态。方法 参照Everson等的方法 ,将SD大鼠予以 72h剥夺睡眠处理后 ,断头取血 ,比色法测定血浆丙二醛 (MDA)和还原型谷胱甘肽 (GSH)的含量以及谷胱甘肽过氧化物酶 (GSH Px)、谷胱甘肽还原酶 (GSH R)和超氧化物岐化酶 (SOD)的活性。结果 与对照组相比 ,睡眠剥夺大鼠血浆MDA的含量增高 ,GSH含量降低 ,GSH Px和GSH R活性降低 ,SOD活性有降低趋势。结论 72h睡眠剥夺大鼠处于氧化应激状态 ,氧化应激可能是睡眠剥夺引起病理变化的机制之一。
Objective To observe oxidative stress of rats induced by 72h sleep deprivation. Methods Using a modified method of Everson et al, male SD rats were sleep deprived for 72h, and contents of plasma malondialhyde (MDA) and glutathione (GSH) and activity of plasma glutathione peroxidase (GSH-Px), glutathione reductase (GSH-R) and superoxide dismutase (SOD) were measured. Results Compared with the control group, plasma MDA of experimental rats increased, and GSH decreased. After 72h sleep deprivation, both GSH-Px and GSH-R activity were reduced, and SOD activity showed a tendency to decrease. Conclusion 72h sleep deprivation promotes oxidative stress, which may be a mechanism mediating injuries by sleep deprivation.
出处
《中国行为医学科学》
CSCD
2003年第5期500-500,519,共2页
Chinese Journal of Behavioral Medical Science
关键词
睡眠剥夺
氧化应激
大鼠
Sleep deprivation
Oxidative stress
Rat
