提高水稻花培再生绿苗(H_1)育种利用率的探讨

RESEARCH ON RAISING UTILIZATION RATE OF REGENERATED GREEN PLANTS DERIVED FROM ANTHER CULTURE IN RICE BREEDING

本文报道了水稻花药培养的绿苗自然加倍率,以抽穗期、株高、穗长三个农艺性状为指标,估算了花培再生绿苗(H_1)群体的育种利用率。结果表明:84-11和选二正反交F_1花药培养的绿苗自然加倍率分别为46％和50％。上述三个性状的育种利用率为:F_1花培绿苗分别为29.27％、29.88％和42.86％;F_2分别为18.64％、28.93％和38.84％;组合(8204/B407)F_3花培绿苗分别为81.00％、72.00％和88.00％。综合三个性状估算育种利用率结果是:花培绿苗率,F_1为3.75％;F_2为2.04％;F_3为50.00％。

This paper reports the natural doubling rate of green plants derived from rice anthers, and based on three agronomic traits (heading date, plant height and panicle length), the breeding utilization rate of regenerated green plants (H_1) derived from anther culture was estimated. The results showed that natural doubling rates of regenerated green plants derived from F_1 anther culture of reciprocal crosses of 84-11 and Xuan Er were 46% and 50%, respectively. For the above three traits, one-trait utilization rate of green plant from F_1anther culture was 29.27% ,29.88% and 42.86%, respectively, that of F_2 was 18.64%, 28.93% and 38.84%,respectively, and that of green plants derived from F_3 anther culture of the cross (8204/B407)were 81% ,72% and 88%, respectively. The synthetical utilization rate of the three traits was 3.75% for green plants from F_1 anther culture, 2.04% for F_2 and 50% for green plants from F_3 anther culture.