长期培养的急性粒细胞白血病患者骨髓贴壁细胞中src原癌基因的高水平表达

High Expression Level of src Oncogene in the Adherent Layer Cells in Long-Term Bone Marrow Culture from Patients with Acute Myeloid Leukemia

在人骨髓细胞长期培养中,观察造血细胞与基质细胞的关系,检测不同类型的白血病患者及其他非血液病患者骨髓贴壁细胞中src原癌基因的mRNA表达水平。基质细胞培养采用人骨髓细胞长期培养的方法,mRNA表达的检测采用地高辛标记cDNA探针和细胞原位斑点杂交法。培养4-6周时,部分急性粒细胞白血病(AML),慢性粒细胞白血病(CML)及骨髓增生异常综合征(MDS)患者的造血细胞获得独立生长的能力。与对照组相比,AML患者骨髓贴壁细胞中src原癌基因表达明显增高(P<0.05)。src原癌基因的高表达,提示在白血病发病中有造血微环境异常。

In order to observe the relationships between the stromal cells and the hematopoietic cells, and the level of src oncogene expressed in the adhesive layer cells of different types of leukemia, human long-term bone marrow culture (HLTBMC) was used. We cultured the bone marrow stromal cells by HLTBMC. The mRNA expression was tested by DIG-labeled cDNA probe and cell in situ dot blot hibridization. When the bone marrow was cultured for 4 - 6 weeks, part of the hematopoietic cells in the bone marrow of AML, CML, MDS patients gained the ability of growing independently. The src oncogene expression level in the adhesive layer cells of AML patients was higher than that of the normal control (P<0.05). The overexpression of src oncogene suggested that the microenvironment abnormality may be relaled with the leukemogenesis.