职业接触敌枯双的细胞遗传学效应的研究

CYTOGENOTOXIC EFFECTS OF OCCUPATIONAL EXPOSURE TO N, N'-METHYLENE-BIS(2-AMINO-1, 3, 4-THIADIAZOLE)

选择生产敌枯双工人11名(男2、女9),接触浓度平均为0.45mg/m^3,年限为4～8年,研究职业接触敌枯双后细胞遗传毒理学效应。结果表明,接触组和对照组(14名)外周血淋巴细胞体外SCE和微核发生率无明显差异。敌枯双在体外对人淋巴细胞的UDS(程序外DNA合成)有诱导作用,呈剂量-反应关系。等量烟酰胺与敌枯双和细胞共培养,前者可逆转敌枯双对DNA损伤的作用。推测敌枯双在体外诱发细胞遗传毒性作用,而在接触工人中此种现象不明显,主要原因可能是作用剂量和体内烟酰胺拮抗作用的结果。

The study was designed to investigate oytogenotoxio effects of occupational exposure to N, N'-methylene-bis (2-amino-1, 3, 4-thiadiazole) (MATDA) in 11 workers (2 male, 9 female) exposed to an average level of 0.45mg/m3 MATDA for 4-8 years. No significant differences in the incidence of SOE and mioronuolei in peripheral lyphocytes was observed between the exposed group and a control group of 3 male and 11 females. MATDA can damage DNA and induce unscheduled DNA synthesis (UDS) in human lymphocytes in vitro, on a dose-effect relationship. Equal concentrations of niootinamide(antagonist of MATDA) added to human lymphocytes culture showed that TJDS induced-MATDA could be prevented by niootinamide. No evidenceof oytogenotoxio effects of occupational exposure were obtained. This might be due to lower levels of exposure in the working population and antagonistic action of niootinamide in foodstuff.