糙皮侧耳(Pleurotus ostreatus)的AFLP指纹图谱分析

AFLP Fingerprinting Map Analysis of Pleurotus ostreatus

在对糙皮侧耳 (Pleurotusostreatus)的AFLP分析条件进行优化的基础上 ,利用该技术建立了 14株产自不同地区的糙皮侧耳DNA指纹图谱 ,并进行了数据分析。结果表明 ,在合成的 14条引物的不同组合中 ,引物对E 3 M 3可以产生较多的DNA多态片段 ,E AGC M CAT引物对的扩增效果最好 ,共获得 184条DNA扩增带 ,其中多态性条带 10 1条 ,占 5 4 89%。利用UPGMA法对所获数据进行聚类分析 ,计算得到糙皮侧耳菌株之间的遗传距离 ,发现不同品种间遗传距离差异较大 ,从 0 192到 0 75 4 ,说明糙皮侧耳的遗传多样性比较丰富。绘制的指纹分析树状图表明 ,14个糙皮侧耳菌株被分为 6个组群 ,其中P17和杂 3的相似性系数最高 ,达到了 81 2 % ,而侧

AFLP analysis was carried out with 14 Pleurotus ostreatus strains from different areas.Optimal conditions of the AFLP fingerprinting analysis for P.ostreatus were first tested and the results showed that the primer pairs E 3/M 3 especially E AGC/M CAT and E AGC/M ACC could give more amplified DNA fragments than others like E 2/M 1 or E 2/M 3.From the fingerprinting map of the primer pair E AGC/M CAT,184 clear and stable DNA bands were observed,including 101 polymorphous bands that are accounted for 54 89%.The genetic similarity coefficient and genetic distance were calculated from AFLP data among 14 P.ostreatus strains.The genetic distance between these strains ranged from 0 192 to 0 754,indicating that P.ostreatus was rich in genetic diversity.UPGMA cluster analysis was also performed.It's shown that 14 P.ostreatus strains are divided into six groups,and strains from same areas or with similar best growth temperature usually have higher comparability in the UPGMA tree. P.ostreatus P17 and P.ostreatus Za3, P.ostreatus Min31 and P.ostreatus Yiping have intimate genetic relationships with each other respectively which were consistent with its geographical distribution and best growth temperature. P.ostreatus Ce5 showed remarkable genetic differentiation,which has farther genetic relationships compared with other P.ostreatus strains.In addition,the reasons for cluster results of strains from AFLP data consistent with morphology,geographical distribution and optimal conditions of AFLP fingerprinting analysis for P.ostreatus were discussed.