原发性肝癌及其他肝病组织中谷胱甘肽硫转移酶的检测

DETECTION OF GLUTATHIONE S-TRANSFERASE INHUMAN HEPATOCELLULAR CARCINOMA ANDOTHER CHRONIC LIVER DISEASE TISSUE

运用免疫组织化学方法,对23例肝癌和14例慢性肝病组织中的人胎盘型谷胱甘肽硫转移酶(GST-π)作原位检测,并以γ-谷氨酰转肽酶(γ-GT)的检测为对照。结果显示:肝癌组织的GST-π阳性检出率(95.7％)高于慢性肝病组织(71.4％),而在9例非肝病对照肝组织内均为阴性;此外,肝癌组织的GST-π阳性率显著高于γ-GT阳性率(p<0.01)。说明GST-π可能是人体慢性肝病,尤其是肝癌的一种敏感的标志酶。

Using the Immunonistochemioal method, expression of the human placenta form of glutathione s-transferase (GST-π) was investigated in liver tissue from 37 oases of hepatocellular carcinoma (HCC) or chronic liver diseases (CLD), including ohronio active hepatitis, cirrhosis, and from 9 oases of non hepatic diseases as controls. In addition, r-glutamyl transpeptidase (r-GT) was also determined by the same method for comparison. Results showed that positive rates of GST-π was higher in HCC group (95.7%) than in CLD group (71.4%), but negative in all control oases. In comparison, the positive rates of r-GT was much lower than these of GST-π in both HCC and CLD (P<0.01). Thus, our work suggests that GST-π to be a more sensitive and stable marker enzyme for in situ human hepatic lesion tissue, especialy HCC.