导向治疗后图象分析肝癌细胞核DNA含量

A STUDY OF THE DNA CONTENT OF HEPATOCELLULAR CARCINOMA AFTER TRAGETING TREATMENT

用图象分析技术测定正常肝、导向治疗后及未经治疗肝癌DNA含量共44例。结果发现二低异倍体(LAN)多见于导向治疗后病人;而高异倍体(HAN)则多见于未经治疗病例(P<0.05)。正常肝、治疗组与未治疗组的DNA含量υ值中位数分别为1.38、2.33和3.30。结果提示:HAN肝癌具有较高的放射敏感性,DNA含量高的癌细胞可能对射线致死性染色体损伤的敏感性也高。事实证明:DNA含量分析可为估价肝癌病人疗效和预后提供客观的定量参数。

DNA contents were measured in normal liver(9 cases), HCC after targeting treatment(17 cases) and HCC without treatment (18 cases) by Image analysis. According to their ploidic levels of DNA, patients with HOO were divided into two groups: low aneuploidio (LAN) and high aneuploidic (HAN). The results of this study showed that the patients after targeting treatment were more of LAN than those without (66.7%VS. 33.3%); also the former was lower of HAN than the latter (29.4% VS. 70.6%) (P<0.05). The median U value in normal liver, HOO after targeting treatment and HCC without treatment were 1.38, 2.33 and 3.30 respectively. The study suggests that HCC of HAN might be more radiosensitive than those of LAN. Possibly the sensitivety of cell with high DNA content to irradiation is higher, resulting in the lethal chromosomal damage.