急性白血病药敏试验与临床缓解的回顾性研究

The retrospective study on drug sensitive test and clinical remission of acute leukemia

本项研究为寻找快速、经济、简便、可靠的白血病药敏试验方法,对MTT法检测的85例初治急性白血病体外药敏试验与临床实际缓解的关系进行了回顾性研究。结果表明:总阳性符合率为70％,阴性符合率为80％,其中AML阳性符合率73％,阴性符合率70％,ALL阳性与阴性符合率分别为61％和100％,AML阳性符合率高于ALL,但无统计学差异(P>0.05);AML和ALL组中白血病细胞对一种化疗药物敏感率分别为71％和86％;AML各亚型临床符合率为67％-100％,ALL各亚型为64％-100％,均无统计学差异(P>0.05)。分析实验结果与临床实际不符的病例,药敏与CFU-L同步检测的16例中12例有白血病集落自发性生长。结论提示MTT药敏试验可以指导临床用药,是一项值得在基层医院推广的检测方法。

In order to find rapid, economical, simple and reliable method of drug sensitivity for acute leukemia, the retrospective study on relationship between drug sensitivity and clinical remission rates in 85 cases with acute leukemia treated first time was performed by MTT method in vitro. Results showed that the total positive coincidence rate was 70 % and the negative coincidence rate was 80 % . The positive coincidence rate and negative coincidence rate of AML were 73% and 70%, respectively. Meanwhile the positive coincidence rate and negative coincidence rate of ALL were 61% and 100%, respectively. The positive rate of AML was higher than that of ALL, but there was no statistical difference (P >0. 05). The sensitive rate of cells of AML and ALL to one kind of chemotherapeutic drug was 71% and 86%, respectively. The positive coincidence rates of subtypes of AML were 67% - 100%, and ones of subtypes of ALL were 64% - 100%, but there was no statistical difference (P > 0. 05). The uncoincidental results of experimental report and clinical condition were analysed. According to experimental results of drug sensitive test and CFU-L assay simultaneously, 12 cases from 16 cases showed independent colonies and clusters growth type. It was concluded that the MTT drug sensitive test may be used directively for drug therapy, and could provide an extensively used test for patients with leukemia in vast hospitals.