一种用于免疫组织化学检验的骨髓活检标本保存的新方法(英文)

A new method of bone marrow biopsy specimen preservation for immunohistochemistry

为进行骨髓活检标本免疫组织化学检验,研究了冰冻置换低温塑料包埋技术处理骨髓活检标本的新方法。结果表明:此技术能良好地保持骨髓组织细胞形态,各种血液细胞抗原成分,尤其是敏感的淋巴细胞抗原保存良好,定位准确,没有弥散,没有背景染色。此方法适用于骨髓活检标本塑料切片的免疫组织化学检验,另外,也可用于处理其它穿刺和内镜活检标本的处理。

In order to perform immunohistochemistry on bone marrow biopsy specimen sections, the new method of freeze-substitution and subsequent low-temperature plastic embedding was studied. The results showed that the morphologic preservation of bone marrow tissue is excellent and all antigens of blood cells, especially labile lymphocyte surface markers were well preserved, which had an accurate localization, no diffusion, and absence of background staining. This is an ideal method for bone marrow biopsy specimen preservation for immunohistochemistry. It is also suitable for processing of needle and endoscopic biopsy specimens.