重组人白细胞介素3对环磷酰胺所致犬造血损伤的治疗作用

Therapeutic effect of rhIL-3 on hematopoietic depression in dogs induced by cyclophosphamide

白细胞介素3(interleukin-3,IL-3)又称多集落刺激因子(multi-colony stimulating factor),是作用于较早期阶段的具有广谱效应的造血刺激因子,对恶性贫血、再生障碍性贫血、骨髓增生异常综合征(MDS)以及各种继发性造血功能障碍疾病有显著疗效。本实验建立环磷酸胺(Cy)致家犬造血抑制的动物模型,用rhIL-3和碳酸锂(LC)进行对比研究,阐明rhIL-3的疗效、作用机理及毒副作用,为进一步研制和应用IL-3提供依据。

Cyclophosphamide (Cy) could decrease the number of peripheral WBC and induce the hematopoiesis depression in dogs. In this study, different doses of rhIL-3 were administrated subcutaneously to the Cy-treated dogs for seven days, and the changes of symptoms and general conditions, peripheral blood cells and bone marrow cells were observed in treated dogs. The results showed that, after rhIL-3 treated, the general conditions improved dramatically, the decreasing degree of the peripheral WBC which was caused by using Cy was alleviated obviously and the duration of low-number WBC (lower than 50% of normal value) was shortened. It was also demonstrated that the bone marrow CFU-GM, CFU-E and BFU-E were promoted, the total number of bone marrow nucleated cell and the counts of platelet and reticulocyte were higher than PBS control group. These effects of rhIL-3 were more obviously in large dose group. It is suggested that rhIL-3 has a strong promoting effects on hematopoiesis and could be used in treatment of hematopoiesis depression especially in the patients using Cy to cure cancer.