医用深低温保存袋的研究

STUDIES ON A MEDICAL FREEZING BAG

本文报道一种深低温(-196℃)生物细胞及组织保存袋。该袋在液氮内保存2年后经37～40℃融冻复温无破损,细胞计数84.5％～91.5％,拒染反应61.3％～104.4％,粒系定向干细胞培养(CFu-Gm)83.9％～164％,材料的理化性能、生物学毒性均适合于应用。

A freezing bag for preservation of biologic cells and tissues at low temperature (-196℃) is introduced. After stored fetal liver cells in bags for two years in liquid nitrogen then thawed to 37-40℃, the bags did not rupture, cell count and percentage of staining rejection decreased to 84.5% and 61.3% respectively, and colony forming unitgranulocytoc/macrophage (CFU-GM) culture 109.4%. The material of bag had good physicochemical properties and low biologic toxicity.