反相高效液相色谱法测定不同产地虎杖中白藜芦醇苷的含量

Determination of the Contents of Polydatin in Rhizoma Polygoni Cuspidati from Different Growing Areas by RP-HPLC

目的 :考察广西各产地虎杖的品质。方法 :采用反相高效液相色谱法测定虎杖中白藜芦醇苷的含量。色谱柱为Nova -PakC18 柱 ,流动相为乙腈 -水 (20∶80) ,检测波长为305nm。结果 :虎杖中白藜芦醇苷含量最高达到3.534mg/g,最低为1.819mg/g。结论

OBJECTIVE:To investigate the quality of Rhizoma Polygoni Cuspidati from different growing areas in Guangxi.METHODS:RP-HPLC method was used to determine the contents of Polydatin in Rhizoma Polygoni Cuspidati,with Nova-Pak C 18 as stationary phase,a mixture of CH 3 CN-H 2 O(20∶80)as the mobile phase,and detecting wavelength was305nm.RESULTS:The contents of Polydatin in Rhizoma Polygoni Cuspidati were variable from3.534mg/g to1.819mg/g.CONCLUSION:The quality of Rhizoma Polygoni Cuspidati from different growing area in Guangxi is dissimilar.