肿瘤转移抑制基因编码蛋白在癌患者外周血中的表达

The Measurement of the Expression Level of Tumor Metastasis-suppressor Genes Coding Protein in Peripheral Blood Cells of Patients with Malignant Carcinoma

目的 探讨肿瘤转移抑制基因编码蛋白在癌患者外周血细胞中的表达规律。方法 采用流式细胞术对 173例各种癌患者外周血nm2 3 +、DCC +、p16+和p5 3V +细胞检出率进行检测。结果 恶性肿瘤患者外周血nm2 3 +、DCC +和p16+细胞检出率均显著低于正常对照 (P <0 0 1或P <0 0 5 ) ,但分布各有不同 ;而p5 3V +细胞检出率显著高于正常对照组 (P <0 .0 5 )。癌转移者外周血nm2 3 +、DDC +和p16+的表达水平显著低于未转移者 (P <0 .0 1)或P <0 .0 5 ) ,而p5 3V则相反。结论 恶性肿瘤能使患者外周血细胞的肿瘤转移抑制基因抑编码蛋白表达水平出现明显异常 ,而这种异常表达与恶性肿瘤转移的关系十分密切 ;不同肿瘤患者异常表达的肿瘤转移抑制基因类型有一定的倾向性。

Objective To study the expression regularity of tumor metastasis-suppressor genes coding protein in peripheral blood cells from patients with malignant carcinoma.Methods The expression rates of nm23+,DCC+,p16+ and p53V+cells in peripheral blood of 173 patients with malignant carcinoma were measured by flow cytometry.Results The expression rates of nm23+,DCC+ and p16+cells in peripheral blood of patients with malignant carcinomas were lower than those of normal control groups with various distribution( P <0.01 or P <0.05),while the expression rate of p53V was significantly higher than the later ( P <0.01).The expression rete of nm 23+,DCC+,p16+cells of patients with metastasis were lower than those of patients without metastasis( P <0.01 or P <0.05),but the expression rate of P 53 V was contrary( P <0.01).Conclusion Malignant tumors may induce the abnormal expression of tumor metastaisis-suppressor gene in peripheral blood cells from patients with malignant tumor,which are closely related to tumor metastasis.The kinds of tumor metastais-suppressor gene expression are different according to the kinds of tumors.