急性一氧化碳中毒对大鼠脑循环的影响

Effect of acute carbon monoxide poisoning on brain microcirculation of rats

目的 探讨急性一氧化碳中毒后脑内血液流变学的变化 ,以进一步弄清迟发性脑病的发病机制 ,为解决其有效防治提供新的线索。方法 体重 2 40～ 2 80g雄性SD大鼠腹腔间断注射染毒 ,分别于染毒后 1、 3、 7、 14、 2 1d颈静脉取血监测血液流变学特性 ,同时检测血浆Fib、ET 1、MDA、NO及cGMP含量。结果 染毒后脑循环血液流变学特性发生明显改变 ,粘度增加 ,且见血管内皮严重受损 ,缩血管物质增加 ,NO cGMP系统受到抑制。结论 急性一氧化碳中毒对大鼠脑循环存在持续性影响 。

Objective To study the hemorheological changes occurred during acute CO poisoning,thereby clarify the pathogenesis of the delayed encephalopathy by acute CO poisoning and further find some new clues for effective prevention and treatment of the delayed encephalopathy.Method Male SD rats,BW 240-280 g,were ip injected with CO repeatedly,then dynamically monitored the hemorheological changes with cervical vein blood such as whole blood viscosity,plasma viscosity,RBC hematocrit etc at 1,3,7,14,21 days after the injection of CO,respectively;while the levels of fibrinogen,endothelin 1,MDA,NO and cGMP in plasma were also measured.Result The hemorheological features were obviously changed,such as the increase of viscosity;besides,the damage of endothelium,the increase of vasoconstrictive substances and the inhibition of NO cGMP system were also seen in the study.Conclusion The acute CO poisoning has a continuing negative effect on brain microcirculation,which might be an important impact factor in the pathogenesis of the delayed encephalopathy by acute CO poisoning.