外源性一氧化碳对大鼠脑内源性气体信使系统的影响

Effect of exogenous carbon monoxide on endogenous gaseous messenger system in brain of rat

目的 探讨外源性一氧化碳对脑内气体信使系统的影响 ,为迟发性脑病机制的研究提供新思路。方法 体重 2 40～ 2 80g雄性SD大鼠腹腔单次注射染毒 ,分别于染毒前及染毒后 0 5、 1、 2、 4、 8h ,颈静脉取血测定血浆NO及cGMP含量 ;取双侧脑海马组织 ,测定HO、NOS活性和cGMP含量。结果 染毒后脑循环中NO cGMP通路一过性上调 ,随后抑制 ;脑海马组织中HO、NOS活性下降和cGMP含量降低。结论 急性一氧化碳中毒可引起体内气体信使系统功能失衡 。

Objective To explore the effect of exogenous carbon monoxide on endogenous gaseous messenger system in brain,thereby to find a new clue for pathogenesis study of the delayed encephalopathy by acute CO poisoning.Method Male SD rats,BW240-280 g,were ip injected with CO repeatedly,then the levels of NO and cGMP in cervical vein blood were dynamically monitored;while the activities of HO,NOS and the level of cGMP in Hippocampi of rats were also measured.Result After injection of CO,NO cGMP level in brain circulatory plasma obviously decreased following a transient increase;the activities of HO or NOS,and the cGMP level in hippocampi were also decreased.Conclusion There was some disequilibrium between gaseous messenger system during acute CO poisoning,that might be one of the most important pathophysiological mechanisms for brain damage by acute CO poisoning.