对苯二甲酸对NIH-3T3细胞间隙连接通讯功能的影响

Effect of terephthalic acid on the function of gap junctional intercellular communication of NIH 3T3 cell

目的 研究对苯二甲酸 (TPA)对NIH 3T3细胞间隙连接通讯 (GJIC)功能的影响。方法 采用噻唑蓝(MTT)比色法及划痕标记示踪技术 (scrape loadinganddyetransfer,SLDT) ,以 0、 12 5、 2 5 0、 5 0 0、 10 0 0、 2 0 0 0 μg/ml的剂量染毒细胞 ,同时设立溶剂对照及阳性对照 ,观察TPA对GJIC的影响。结果 TPA对细胞GJIC功能抑制随着染毒剂量增大和染毒时间延长而逐渐增强 ,呈现剂量 效应和时间 效应关系 ,在 2 5 0 μg/ml或更高剂量染毒 1h或更长时间时 ,细胞GJIC功能被明显抑制 ,在染毒 8h时各剂量组细胞GJIC功能抑制效应均最显著 ;去除TPA后 ,GJIC功能有恢复倾向 ,但在 12h内仍不能恢复至正常水平 ;加入哺乳动物肝脏微粒体酶 (S9)能加重TPA对细胞GJIC功能抑制作用。结论 TPA原形及其代谢产物对NIH

Objective To assess the effect of TPA on GJIC function of NIH 3T3 cell.Method TPA was added to NIH 3T3 cell suspensions with the doses of 0,125,250,500, 1 000 and 2 000 μg/ml respectively 24 h after culture,MTT assay and SLDT assay were used for the detection of GJIC function.Result TPA could inhibit GJIC function of NIH 3T3 cell,which was not only dose dependent, but also time related;even at a dose of 250 μg/ml TPA,the obvious inhibition of GJIC function could still be observed 1 hour after administration,and the maximum inhibition occurred at 8 h after treatment of TPA.Although the inhibition of TPA on GJIC function of NIH 3T3 was reversible after ceasing exposure to TPA,but it still kept a lower level within 12 h after administration.The study also showed that the inhibition of TPA on GJIC function could be enhanced by the adding of S9(microsome enzyme of mammal liver).Conclusion These results suggested that either TPA or its metabolites could inhibit the GJIC function of NIH 3T3 cells.