对几种原代细胞彗星实验敏感性的探讨

The studies on selection for sensitive primary cells used in the comet assay

目的 筛选一种动物原代敏感细胞用于彗星实验 ,以便更广泛地监测环境样品的遗传毒性。方法 用重铬酸钾 (K2 Cr2 O7)和过氧化氢 (H2 O2 )作为受试物 ,对小鼠的肝、脾、肾细胞进行彗星实验 ,观察K2 Cr2 O7和H2 O2 对这 3种细胞造成的DNA损伤 ,从而判断细胞的敏感性。结果 以K2 Cr2 O7染毒 ,肝细胞的检测阈值 ( 10nmol/L)低于脾和肾 ( 10 0 0nmol/L)。以H2 O2 染毒 ,在相同浓度下肝细胞迁移长度最大。结论 肝细胞在彗星实验中的敏感性最高、自身活化能力强 ,且取材方便、耗时短、费用低 ,可以作为一种敏感细胞应用于彗星实验 。

Objective In order to select sensitive primary cells to be used in the comet assay to detect genotoxicity for environmental samples.Method Mice were exposed to varied concentrations of potassium dichromate(K 2Cr 2O 7)and hydrogen peroxide(H 2O 2)and their cells were isolated from the liver,spleen and kidney to detect the effects of DNA damage using comet assay to find sensitive ones.Result Threshold for detection of DNA damage in the liver cells(10 nmol/L) was less than that in the spleen and kidney cells (1 000 nmol/L),as exposed to K 2Cr 2O 7.The longest migration length of DNA was found in the liver cells as exposed to the same concentration of H 2O 2.Conclusion The liver cells had the highest sensitivity and auto activation in the comet assay,with features of convenience,quickness,and low cost,and can be selected as target cells in the comet assay to detect genotoxicity for environmental samples.