镍冶炼烟尘对中国仓鼠肺细胞毒性作用的实验研究

The toxic effect of nickel refining dusts on lung fibroblasts of Chinese hamsters

目的 观察镍冶炼烟尘对中国仓鼠肺细胞 (CHL)的毒性作用。方法 以我国某镍冶炼厂电炉烟道尘为受试物 ,采用噻唑蓝 (MTT)比色法检测CHL细胞活性。结果 不同浓度下的镍冶炼烟尘 ( 6 2 5、 12 5 0、 2 5 0 0、 5 0 0 0、10 0 0 0 μg/ml)作用一定时间后 ,CHL细胞存活率下降 ,呈现出时间 剂量 反应关系。接触受试物 3 6h ,细胞生长半数抑制浓度IC50 为 2 1 3 6μg/ml。同时镜下观察细胞形态也发生改变。 结论 镍冶炼烟尘可以抑制CHL细胞生长 。

Objective To observe the toxicity of nickel refining dusts on CHL(Chinese hamster lung cells,CHL).Method MTT colorimetric assay was used for detecting of CHL activity.Result The survival rates of CHL were obviously decreased under the exposure of various concentrations(6 25?12 50?25 00?50 00?100 00 μg/ml)of nickel refining dusts,which was dose time dependent and the 50% inhibited concentration for cell growth was 21 36 μg/ml,while the morphological alterations could be observed,too.Conclusion Nickel refining dusts might inhibit the proliferation and the metabolic activity of mitochondria of CHL.