摘要
目的 :探讨内毒素血症对大鼠脑内促甲状腺激素释放激素 (TRH)与其受体含量及分布情况的作用。方法 :建立内毒素血症加失血性休克双重打击模型 ,用放射免疫法测定模型动物脑内 TRH及其受体含量。结果 :在内毒素血症早期 ,脑内 TRH含量显著减少 ,TRH受体总量增加 ,但与 TRH亲和力下降 ,表现为可结合 TRH的受体无显著性变化。结论 :脑内 TRH含量与外周不平行 ;内毒素血症早期脑内 TRH减少及有活性TRH受体含量稳定可能与机体对脑组织的保护作用及遏制多器官功能障碍综合征进展有关。
Objective: To study the dose and location of thyrotropin releasing hormone(TRH) and its receptor in Wistar rat's brain when early endotoxemia happened. Methods: Twenty-two Wistar rats were selected. In 13 mouse the model was established with endotoxemia and hemorrhagic shock.The TRH and its receptor were observed with radioimmunoassay(RIA), and were compared to the 9 control rats. Results: The dose of TRH decreased significantly in the early stage of endotoxemia. The maxium dose(Bmax) of TRH increased while Kd (affinity) decreased,so the effective TRH receptor had no significantly change. Conclusion:The dose of TRH is different in brain and serum. The decrease of TRH and the steady of active TRH receptor may protect the brain tissue and repress multiple organ dysfunction syndrome(MODS) in the early stage of endotoxemia.
出处
《中国危重病急救医学》
CAS
CSCD
2003年第10期622-624,共3页
Chinese Critical Care Medicine
基金
黑龙江省教育厅课题资助 ( 95 5 10 66)
