烫伤合并绿脓杆菌感染大鼠红细胞趋化因子受体结合活性的变化

Change of the erythrocyte chemokine receptor binding activity in scalded rats with pseudomonas aeruginosa infection

目的 :探讨感染对烫伤大鼠红细胞趋化因子受体 (ECKR)结合活性的影响。方法 :SD大鼠随机分成假烫伤组和烫伤感染组及单纯感染组。烫伤感染组大鼠行总体表面积 (TBSA) 30 % 度烫伤 ,用 ATCC 2 785 3细菌菌液制备感染模型 ;应用酶联免疫吸附法 (EL ISA)以白介素 8(IL 8)为配体检测大鼠红细胞趋化因子受体的结合活性。结果 :与假烫伤组比较 ,烫伤和未烫伤感染大鼠 ECKR结合活性均呈极显著性下降 (P均 <0 .0 1)。与感染后 6 h比较 ,感染后 8h大鼠 ECKR结合活性两组均呈显著性上升 (P均 <0 .0 1)。烫伤感染组与单纯感染组比较 ,烫伤感染组 2 h与 8h两个时间点 ECKR结合活性均显著低于单纯感染组 (P均 <0 .0 5 )。烫伤感染组下降幅度较大 ,且最低 (2 h)出现时间比感染组 (6 h)早。结论 :感染可进一步加速烧伤大鼠 ECKR结合活性的下降 ,在抗感染的过程中红细胞可能参与体内趋化因子家族的调控。

Objective:To explore the influence of infection on the erythrocyte chemokine receptor (ECKR)binding activity in severely scalded rats. Methods:The Sprague-Dawley (SD) rats were randomly divided into three group: sham scald group (A), burn and infection group(B) and infection group(C). The B group rats were scalded with 30% total body surface area (TBSA) of Ⅲ degree, and the rats ECKR binding activity with interleukin-8 (IL-8) as ligand were detected by enzyme-linked immunosorbent assay(ELISA) at 1, 2, 4, 6 and 8 hours after infection. Results:Compared to that in A group, ECKR binding activities declined significantly (both P<0.01) after infection in both of B and C groups, but they increased at 8 hours (P<0.01) . ECKR binding activity in B group was significantly less at 2, 8 hours than that in C group after infection (both P<0.05). The declining range of ECKR binding activity was more in B group, in which the decline of ECKR binding activity appeared earlier (2 hours) than that in C (6 hours) group. Conclusion:The infection lead to the decline of ECKR binding activity in burned rats, and the erythrocytes might participate in the chemokine regulation and play a novel role in the infection.