摘要
目的 :探讨茶多酚防治糖尿病肾病的机制。方法 :复制糖尿病肾病大鼠模型 ,随机分为 5组 :单侧肾切除组 (C组 )、糖尿病肾病模型对照组 (DC组 )、茶多酚提前治疗组 (B组 )、同时治疗组 (I组 )和滞后治疗组 (A组 )。分别于造模后 0、2、4周 ,检测血糖、2 4h尿蛋白 ,观察肾皮质转化生长因子 (TGF β1)基因和蛋白的表达。结果 :茶多酚有效降低了早期糖尿病肾病大鼠 2 4h尿蛋白 ,抑制了肾皮质中TGF β1mRNA和蛋白质表达的升高 ,尤以茶多酚提前治疗组效果最为明显。结论 :在糖尿病肾病早期使用茶多酚治疗 ,可以通过降低肾皮质TGF β1基因与蛋白的表达 ,有效延缓了糖尿病肾病的发生和发展。
Objective:To explore the mechanism of action of tea polyphenols(TPs) in the prevention and treatment of early diabetic nephropathy.Methods:Setting up the model of diabetic nephropathy(DN)in rats and then dividing them into five groups:(1)control with resection of one kidney;(2)DN control;(3)DN with treatment of TPs;(4)DN with synchronous treatment of TPs;(5)DN with deferred treatment of TPs.After the model established,their blood sugar and urinary protein in 24 hours were detected at the time point of 0,2 and 4 weeks.Protein and mRNA of TGF -β 1 in their renal tissues were also studied.Results:TPs treatment decreased urinary protein,restrained the up-regulation of TGF-β 1 in renal tissue and protected against early diabetic glomerular injury in DN rats.Preventive treatment of TPs on DN rats shows the best effects.Conclusion:TPs inhibited TGF-β 1 protein and mRNA over-expression in renal cortex of diabetic rats,suggesting that TPs might play a beneficial role in experimental diabetic nephropathy.Its mechanism may be partly by inhibiting TGF-β 1 protein and mRNA over-expression in kidney.
出处
《中日友好医院学报》
2003年第5期286-289,F004,共5页
Journal of China-Japan Friendship Hospital
