摘要
目的 探讨卵巢癌多药耐药的机制及应用核酶对阿霉素 (ADM)引起的多药耐药 (MDR)的逆转。方法 应用共聚焦激光显微镜 (confocal)、逆转录聚合酶链反应 (RT PCR)、蛋白免疫印迹法(Westernblot)等检测卵巢癌细胞株A2 780及阿霉素耐药株A2 780 /ADM多药耐药基因 (mdr 1)及其编码的p 糖蛋白 (p gp)的表达 ,并比较导入mdr 1特异性核酶后A2 780 /ADM细胞MDR表型的改变。结果 A2 780 /ADM细胞对ADM的耐药性是A2 780细胞的 8.4 3倍。A2 780 /ADM细胞中mdr 1基因和p gp表达较A2 780细胞增高 ,转染mdr 1核酶基因后 ,A2 780 /ADM细胞的mdr 1mRNA和p gp表达明显下降。结论 ADM所致的卵巢癌细胞MDR与mdr 1基因过度表达有关 ,应用mdr 1核酶能在一定程度上逆转A2 780 /ADM细胞的MDR。
Objective To study the mechanism of multidrug resistance and its reversal by mdr-1 ribozyme in human ovarian cancer. Methods The expression of mdr-1 and p-glycoprotein (p-gp) was studied by confocal laser microscope (Confocal), RT-PCR and Western blot analysis in adriamycin-resistant human ovarian cancer cell line (A2780/ADM) and adriamycin-sensitive one (A2780). The mdr-1 ribozyme was transfected into the A2780/ADM by Lipofectamine 2 000 to overcome the multidrug resistance in ovarian cancer. Results The expression of mdr-1 mRNA and p-gp in A2780/ADM was significantly higher than that in A2780. The expression of mdr-1 mRNA and p-gp in A2780/ADM was lowered after being transfected by mdr-1 ribozyme. Conclusion Multidrug resistance of A2780/ADM, possibly being caused by overexpression of mdr-1 gene, can be partially reversed by mdr-1 ribozyme.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2003年第5期425-428,共4页
Chinese Journal of Oncology
基金
国家自然科学基金 (3 9870 765
3 9840 0 0 9)资助项目
国家杰出青年科学基金 (3 0 0 2 5 0 17)资助项目
