乙肝病毒x蛋白激活缺氧诱导因子-1通路促进血管内皮生长因子表达的研究

Hepatitis B x protein activated vascular endothelial growth factor expression through hypoxia inducible factor-1 pathway

目的 探讨乙肝病毒x蛋白 (HBx)是否可以通过激活缺氧诱导因子 1α(HIF 1α)通路促进血管内皮生长因子 (VEGF)的表达。方法 将pIRES EGFP HBx和pTK Hyg质粒共同转染人肝癌细胞系SMMC 772 1,筛选出荧光阳性和阴性的抗性克隆 ,检测两种克隆中VEGF和HIF 1α在mRNA和蛋白水平的表达。结果 荧光阳性细胞有HBx的稳定整合和表达 ,并有HIF 1α和VEGF的上调表达 ;荧光阴性细胞无HBx的表达 ,HIF 1α和VEGF的表达亦为阴性。结论 HBx可以通过激活HIF 1通路促进VEGF的表达。

Objective To invesigate whether hepatitis B x protein(HBx) stimulates vascular endothelial growth factor (VEGF) through hypoxia inducible factor-1(HIF-1α) pathway. Methods Two plasmids including pIRES-EGFP-HBx and pTK-Hyg were co-transfected to a hepatocellular carcinoma cell line SMMC-7721. With fluorescence-positive and fluorescence-negative hygromycin-resistant colonies selected, expressions of VEGF and HIF-1α in protein or/and mRNA level were detected. Results Fluorescence-positive cells were stably integrated with HBx,in which expression of HIF-1α and VEGF were upregulated. Fluorescence-negative cells did not express HBx, VEGF or HIF-1α. Conclusion HBx can activate VEGF through HIF-1α pathway.