舒肝凉血方对体内雌激素影响的实验研究

In vivo effect of Shu-Gan-Liang-Xue decoction on estrogen

目的 观察中药舒肝凉血方对动物体内雌激素的影响。方法 将试验小鼠随机分为对照组、中药组、三苯氧胺 (TAM)组和中药 +TAM组。灌服中药 2 1d后 ,用免疫法检测试验小鼠血清性激素水平变化 ,计算小鼠子宫重量指数 ,同时取病理 ,观察小鼠子宫内膜的变化情况。结果 舒肝凉血方可使实验小鼠血清雌激素水平明显下降 ,与对照组相比 ,差异有非常显著性 (P <0 .0 0 1)。子宫重量指数降低 ,但与对照组相比 ,差异无显著性。病理结果显示 ,中药组与对照组比较 ,子宫内膜无明显改变 ;中药 +TAM组与TAM组比较 ,前者可明显减轻子宫内膜增厚的程度。结论 中药舒肝凉血方可明显降低体内雌激素水平 ,与TAM有协同作用 ;同时可减轻TAM使子宫内膜增厚的副作用。

Objective To observe the in vivo effect of traditional chinese medicine (TCM) Shu-Gan-Liang-Xue (SGLX) decoction on estrogen in vivo in mice. Methods Mice were randomly divided into control, tamoxifen (TAM), SGLX and SGLX +TAM groups. After SGLX decoction had been given to mice for 21 days, the serum hormone level of mice was tested by radioimmunal method, uterine weight index was obtained by uterine weight divided by body weight. Endometrial change was pathologically observed. Results SGLX decoction reduced the level of serum estrogen more than the control with significant difference (P<0.001). Uterine weight index was more lowered in the SGLX group than the control giving a difference but not significant. The endometrium in the SGLX group showed no change when compared with that of the control, but the SGLX + TAM group showed slightly more endometrial hyperplasia than the TAM group. Conclusion SGLX decoction, having synergistic effect on TAM, can reduce serum hormone level and alleviate the endometrial hypertrophy side effect of TAM.