离子束介导甘蓝全DNA转化拟南芥菜的RAPD分析

RAPD ANALYSIS OF Arabidopsis thaliana TRANSFERRED WITH TOTAL DNA OF CABBAGE BY ION BEAM

在离子束介导甘蓝外源全DNA转化拟南芥菜的当代发现了 2株表型变异株 ,以其中的1株 (编号为T6)作为研究对象 ,用 40对 1 0碱基的随机引物对该株和其后代株进行RAPD分析 ,发现S1 68号引物对T6和其后代株的RAPD结果与对照相比有明显差异 ,说明离子束介导外源基因转化可以引起受体基因组变异 ,并且该变异有遗传性。对差异条带测序后同拟南芥菜基因组序列比对 。

Two mutants were found among the Arabidopsis thaliana transferred with total DNA of cabbage. Variation of genome of T6 and its offspring were analyzed by RAPD PCR with 40 random primers. The result from S168 primer was different from the CK, indicating that variation of genome can be made by total DNA transferring by use of ion beam , and this variation is hereditary. It is found that S 168 1850 is included within the gene of ABC transporter by aligning with genome of Arabidopsis thaliana in TAIT.