冬凌草甲素的提取工艺研究

Study on method for extracting and separating oridonin in Rabdosia rubescens

目的 采用近红外光谱 ( NIR)技术对冬凌草中冬凌草甲素的提取工艺进行研究。方法 采用柱色谱法分离冬凌草甲素 ,应用 NIR技术寻找最佳工艺条件。结果 此工艺所得冬凌草甲素纯度较高 ,且样品与对照品的HPL C峰保留时间相同 ,NMR和 IR图谱相同。结论 本法洗脱速度快 ,安全、成本低、方便 ,并且重现性好。

Object To study the most suitable conditions of the extraction separation process of the oridonin in Rabdosia rubescens (Hemsl.) Hara with NIR. Methods The oridonin was chromatographed on a silica column and found the optimum conditions with NIR. Results The oridonin extracted by this method had higher purity. The retention time of the sample was the same as that of the reference substance. The spectra of NMR and IR are the same. Conclusion This method is proved to be fast, safe, lower cost, simple and has good reproducibility.