板蓝根中丁香酸的抗内毒素作用

Anti-endotoxic effects of syringic acid in Radix Isatidis

目的 研究从板蓝根中分离出的丁香酸的抗内毒素作用。方法 将丁香酸配成 1%水溶液 ,鲎试验法做抗内毒素定量测定 ;用内毒素致兔发热实验检测丁香酸体内抗内毒素作用 ;内毒素致小鼠死亡实验测定丁香酸保护作用。结果 0 .334mg/m L丁香酸可使 4 EU内毒素降为 0 .777EU ,破坏率为 83.16 % ;1%丁香酸溶液可使内毒素引起的兔热显著降低 ;使同等剂量脂多糖引起的小鼠死亡率由 6 8%降为 2 0 %。结论 丁香酸有抗内毒素作用。

Object To study the anti endotoxic effect of syringic acid (SA) which was isolated from Radix Isatidis (Banlangen in Chinese, BLG). Methods SA was extracted and isolated from BLG and made to 1% solution. The content of SA pretreated ET was quantitatively determined using Limulus Test. Then the ability of fever induction of endotoxin (ET) pretreated with SA was measured using endotoxin induced fever test in rabbits. At last, the lipopolysaccharide (LPS) induced death in mice pretreated with and without SA was compared. Results Being pretreated with SA, 83.16% ET was destroyed, the ET induced fever in rabbits relieved markedly and the LPS induced death rate in mice dropped from 68% to 20%. Conclusion SA isolated from BLG has anti endotoxic effects.