利用分子标记技术检测玉米杂种纯度研究

Study of Maize Hybrids Seed Purity Test Using Molecular Markers

以 12份玉米自交系及其组配的 8个杂交种为材料 ,利用分子标记技术进行杂种纯度检测研究。采用RAPD标记方法 ,从 30 0个随机引物中 ,筛选确定了 8个杂交种种子纯度检测引物 ,制定了检测标准标记图谱。建立了海禾 3纯度分析的SCAR标记 ,该SCAR标记与RAPD标记在种子纯度检测上结果完全一致。

maize inbred lines and 8 hybrids were analyzed with RAPD marker technique. 300 arbitrary primers were screened and primers were identified for hybrids seed purity test on 8 elite maize hybrids . Standard maps of hybrids seed purity test were given. A SCAR marker was conversed from RAPD marker of Haihe3 maize hybrids.The result of SCAR and RAPD test in Haihe3 hybrids seed purity were identical.