不同补料发酵方式对发夫酵母产虾青素的影响

Influence of Different Fed-batch Culture Methods on the Produce of Astaxanthin with Phaffia rhodozyma

对发夫酵母的不同补料发酵方式进行了研究 ,期望提高发酵产率 ,以便对工业化生产提供一定的指导作用。实验结果表明 :采用恒pH葡萄糖 -氨水流加培养 ,色素量和生物量均具有最大值 ,分别为 5 4 .3μg ml和 4 9.5 μg ml;其次是指数流加培养 ,色素量和生物量分别为 4 9.9μg ml和 4 7.4mg ml;恒pO2 流加方式下色素量和生物量分别为 34.0 4ug ml和 35 .4mg ml;恒pH葡萄糖流加方式所得的色素量和生物量最小 ,分别为 32 .3μg ml和 31.5mg ml。不同的补料发酵方式对发夫酵母生长和色素形成的影响很大。

Studied on the different fed-batch fermentation modes of Phaffia rhodozyma in this article,expect to improve the yield of fermentation in order to have a positive effect on industry production.The results showed that pH-stat glucose-ammonia feeding culture brought about the biggest pigment yield 54.3μg/ml and the biggest dry cell weight 49.5mg/ml; pigment yield reaching 49.9ug/ml and dry cell weight 47.4mg/ml were obtained from exponential feeding culture; pO_2 -stat feeding culture brought about the pigment yield 34.04μg/ml and the dry cell weight 35.4mg/ml;there has the smallest production of pigment and dry cell weight with pH-stat glucose feeding culture,32.3μg/ml and 31.5mg/ml respectively;the production of pigment and dry cell weight had a huge difference due to different feeding culture methods.