甲醛对小鼠睾丸各阶段生殖细胞致突性的研究

The Mutability Study on Formaldehyde to Different Stages of Testis Germ Cells of Mice

目的 观察甲醛 (Formaldehyde)对小鼠睾丸各阶段生殖细胞的致突毒性。 方法 选用雄性昆明小鼠 ,采用腹腔注射染毒 ,染毒的甲醛剂量分别 0 .2 0、2 .0 0、2 0 .0 0 mg/ kg· bw。每天 1次 ,连续 5 d。第 6 d和 15 d分别处死动物进行 SCE和微核试验 ,判断生殖细胞的致突毒性。 结果 甲醛的中、高剂量组早期精细胞微核率显著升高 (P<0 .0 5 ) ;同时精原细胞 CE交换率也显著升高 (P<0 .0 5 )

Objective To study the mutability of formaldehyde on different stages of testics germ cells of mice. Methods Male Kunming mice were used. FA groups had been exposed to FA by ip for 5 days. Formaldehyde′s contaminated dose were 0.20mg/kg·bw, 2.00 mg/kg·bw, 20.00 mg/kg·bw. We killed mice at 6th and 15th day and make the tests of sister chromosome exchanges, and the micronuclear.and judged the mutation of Formaldehyde on germ cells in different stages of male mice. Result There is a significant increase of MN ratio in early spermatogenic cells and SCE ratio in medial and high dose groups (P<0.05).