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酶抑制增强纸片扩散法检测产超广谱β—内酰胺酶细菌中的临床应用

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摘要 Objective To investigate the distribution of producing extended - spectrum β - lacta-mase(ESBLs) bacteria in the hospitals of Yueyang for better comtrol and treatment. Method Producing ESBLs bacteria were detected using standard double - disk spread test. Results Among 227 strains of Escherichia coli 120 strains of Klebsiella pneumoniase producing ESBLs bacteria accounted for 47.5% (108/227) ,57.5% (69/120),The wards isolated rate of ESBLs in the following order:59.4% in ICU wards,47.9% tumoral diseases wards and 23.9% others. Allproducing ESBLs bacteria were susceptible to imipenem. No strain was intermediately resistant.Conclusion This method was simple, credible and cheap. It was suitable for detection of ESBLs routinely in basic clinical micrology laboratory. The main source of producing ESBLs bacteria was ICU. To kill these bacteria imipenem can be used first, and then pipemcilline/tazobactan.
出处 《美国中华健康卫生杂志》 2003年第2期67-68,共2页
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