腭裂与非腭裂小鼠腭突发育的比较形态测量学研究

Comparative morphometrical study on development of palatal shelves in cleft and non-cleft palate mice

目的 通过对 18三体伴腭裂胚鼠和正常胚鼠大体标本腭突发育、冠状序列连续切片中 ,不同特征平面内腭突发育的比较形态测量学研究 ,认识腭裂与腭突发育的量化关系。方法 借助计算机图像处理系统测定 5 2对处于相同或相近发育阶段的腭裂与非腭裂Han NMRI小鼠的大体腭突宽度和横截面积 ,以及 30对胎鼠颅上颌复合体冠状序列连续切片 (厚度 7μm)中腭突宽度及横截面积、腭骨、上颌骨腭突和牙槽突横截面积 ,并估算腭骨在矢状平面的长度。进一步对腭裂及非腭裂组行量化比较。结果 18三体伴腭裂小鼠大体标本腭突的宽度和面积明显小于正常小鼠 ;连续序列切片中腭裂小鼠腭突宽度及横截面积、腭骨、上颌骨腭突和上颌突横截面积以及腭骨长度均明显小于正常小鼠。结论 18三体伴腭裂小鼠腭突在各个方向均呈严重的发育不足 ,这是与鼠胚的

Objective To quantitatively compare the relationship between the congenital cleft palate and development of the palatal shelf Methods Fifty two pairs palatal shelves were macroscopic measured, and 60 series coronal sections were microscopically measured, which were precisely orientated in the coronal plane and serially sectioned at 7 μm thickness With the aid of computer imaging analysis system the widthes and areas of the palatal shelves in vertical and coronal direction, the maximal areas of the palatal bone and palatal process and alveolar process were measured and compared quantitatively between the cleft group and non cleft group Results The widthes and areas of palatal shelves in cleft foetuses showed significant reduction macroscopically and microscopically as well as the maximal areas of the palatal bone,in addition, both of two processes of the maxilla showed significant developmental deficiency Conclusions The palatal shelves show significant developmental hypoplasia in three dimension directions, which have significant correlation between palatal cleft and trisomic condition