低强度微波辐射抑制体外培养兔晶状体上皮细胞增殖的研究

Effect of low-intensity microwave radiation on proliferation of cultured epithelial cells of rabbit lens

目的 比较不同低强度微波辐射对体外培养兔眼晶状体上皮细胞增殖活性及细胞周期的影响 ,初步探讨晶状体上皮细胞对微波辐射损伤的耐受剂量。方法 在兔晶状体上皮细胞体外培养的基础上 ,应用频率 2 4 5 0MHz,功率密度分别为 0 .10、0 .2 5、0 .5 0、1.0 0、2 .0 0mW cm2 的微波连续辐射细胞 8h ,HE染色 ,观察辐射前后细胞形态学改变 ,四唑盐 (MTT)比色法测定细胞增殖活性 ,并用PI荧光染色检测微波辐射对细胞周期时相的影响。结果 0 .5 0、1.0 0、2 .0 0mW cm2 微波组辐射晶状体上皮细胞 8h后 ,均可降低细胞增殖活性 ,使细胞排列紊乱、固缩或脱壁 ,同时抑制细胞DNA合成 ,G0 G1 期细胞百分比分别为 71.95 %± 2 .12 %、75 .6 8%± 3.35 %、82 .4 0 %± 8.6 8% ,高于对照组( 6 1.6 8%± 5 .76 % ) ,差异有显著性 (P <0 .0 5或P <0 .0 1) ,而S期细胞所占比例分别为 19.32 %±3.0 7%、16 .0 8%± 4 .91%、12 .98%± 8.0 8% ,较对照组 ( 2 8.0 5 %± 5 .12 % )明显减少 ,差异有显著性 (P<0 .0 5或P <0 .0 1)。功率密度为 0 .10、0 .2 5mW cm2 的微波组对细胞增殖活性和细胞周期未见影响 ,与对照组的差异无显著性 (P >0 .0 5 )。结论 功率密度高于 0 .5 0mW cm2 的微波连续辐射 8h ,可对体外培养的兔晶状?

Objective To compare the effects of different doses of microwave on the proliferative activity and cell cycle of cultured epithelial cells of rabbit lens,and to investigate the limit tolerant of microwave exposure. Methods Cultured epithelial cells of rabbit lens were exposed to microwave radiation with frequency of 2 450 MHz and power density of 0.10,0.25,0.50,1.00,2.00 mW/cm 2 for 8 h in vitro.HE staining was used to observe the morphological changes of lens epithelial cells,the proliferative activity and cell cycle were measured by MTT assay and PI fluorescent staining. Results 8 h after radiation,0.50,1.00 and 2.00 mW/cm 2 microwave could decrease the proliferation of lens epithelial cells,make the cells disordered arrangement,shrinkage,detachment,and inhibit the synthesis of cell DNA.The percentage of G 0/G 1 phase cells were 71.95%±2.12%,75.68%±3.35% and 82.40%±8.68% respectively,which were higher than that in control group(61.68%±5.76%, P <0.05 or P <0.01).The percentage of S phase cells were 19.32%±3.07%,16.08%±4.91% and 12.98%±8.08% respectively,which were lower than that in control group(28.05%±5.12%, P <0.05 or P <0.01).No obvious changes could be detected in 0.10, 0.25 mW/cm 2 microwave groups( P >0.05). Conclusion Microwave exceeding 0.50 mW/cm 2 may make injury to lens epithelial cells after 8 hour radiation,which may be related to the effect of microwave radiation on cell cycle.