电磁脉冲辐射对小鼠生殖功能的影响

Effect of electromagnetic pulse irradiation on mice reproduction

目的 探讨电磁脉冲辐射对小鼠生殖功能的影响。方法 6～ 8周龄二级昆明种雌性或(和 )雄性小鼠分别于交配前、雌性小鼠受孕后接受 5次 6× 10 4 V m电磁脉冲重复全身辐射 ,在受孕后第 18天活杀 ,用足孕期畸形学指标进行分析。结果 电磁脉冲辐射对大多数雌鼠脏器重量、脏器重 体重比值均无明显影响 ,但雌鼠辐射后受孕或孕后辐射 ,存活仔鼠尾长均缩短 ,与对照组比较 ,差异有显著性 (P <0 .0 5 ) ,后者伴有雄性仔鼠比例明显降低 ( 0 .85± 0 .0 9vs1.0 9± 0 .17) ,差异有显著性(P <0 .0 5 ) ;雄鼠辐射后 ,雄性仔鼠比例 ( 0 .76± 0 .18)明显降低 ,与对照组比较 ,差异亦有显著性 (P <0 .0 1) ;雌雄鼠均辐射后 ,存活仔鼠尾长缩短 ,雄性仔鼠的比例增加。辐射组死胎率均较正常对照组有不同程度的增高 ;除雄性小鼠辐射后胎吸收率降低外 ,其余辐射组均见胎吸收率明显增加 ,与对照组比较 ,差异有显著性 (P <0 .0 5 )。结论 无论是雌或雄性小鼠交配前 。

Objective To evaluate the effect of electromagnetic pulse(EMP) irradiation on mice reproduction. Methods Female/male Kunming mice,6～8 weeks old,prior to mating,or female after pregnancy were treated with whole body irradiation by 6×10 4 V/m electromagnetic pulse(EMP) for five times.The pregnant mice were killed on the 18th days,and teratological markers were analysed. Results EMP irradiation caused no significant changes in most of female organ weight and organ/body weight ratio.But it caused significant shortening in tail length of live foetus in the female mice before conception(prior to mating) or after pregnancy( P <0.05),and obvious decrease in male offspring ratio(0.85±0.09 vs 1.09±0.17, P <0.05).The male offspring ratio also significantly decreased(0.76±0.18 vs 1.09±0.17, P <0.01) after male mice irradiated by EMP.The tail length of live foetus was shortened and male offspring sex ratio was increased after both male and female mice were irradiated by EMP.EMP irradiation also caused a significantly higher fetal death rate than normal control( P <0.05).The embryo absorption rate was increased after irradiation except that was decreased in male mice. Conclusion EMP irradiation has effect on pregnancy and offspring development in both male and female mice before mating and in female mice after pregnancy.