GAP质控下栽培丹参重金属内控标准物的制备和表征

Preparation and Characterization of Radix Salvia Reference M aterial for Heavy Metals under GAP Control

目的 :用GAP质控下栽培的中药丹参作为重金属内控标准物 ,研究其制备和表征方法。方法 :采用四川中江GAP栽培条件下的丹参制备标准物 ,经浓硝酸 过氧化氢微波消解体系处理后用ICP MS测量其重金属含量 ,方法准确、可靠。结果 :用国家一级标准参考物质检验测量方法的准确性和精密度 ,回收率大部分在 90 %～110 % ,精密度小于 5%。结论 :经过不同实验室测试和不同时期稳定性测试的实验结果表明 ,丹参内控标准重金属含量的数据准确可靠 ,稳定性好 ,可作为丹参中药材重金属质量控制的参考标准 。

Objective: To prepare and characterize t he heavy metal reference mat erial of radix salvia planted in Zhongjiang, Sichuan province under the good agr icultural procedure (GAP). Method: After being prepared, t he reference material was digested with 65% HNO 3 and H 2O 2 by microwave-assisted oven systems, and the heavy metals in Radix salvia reference material were accurately determined by inductiv e ly coupled plasma mass spectrometry (ICP-MS). Result: The measuring method was va lidated by running certificated reference materials, including bush twigs and le aves (GBW07602) and tea twigs and leaves (GBW07605) under the same conditions. T he recoveries of the elements mostly ranged from 90% to 110%, and the RSD was wi t hin 5%. The measurement of radix salvia reference material was carried out by di f ferent laboratories with ICP-MS and by several time intervals in one year for t h e stability. The results showed that the concentration of the heavy metals provi ded were accurate and the reference material was stable. Conclusion: The reference material is suitable to be the criterions of heavy metals for radix salvia in t he qualities controlling, and is also suitable to be the criterion of poisonous heavy metals of other herbs in the administration of GAP.