黄芩汤及其肠道菌群的代谢产物对D-半乳糖胺诱导的肝损伤的保护作用的比较研究

A Comparison of the Protective Activity of Liver Injury Induced by D-galact-osamine Between Huangqin-Tang and Their Metabolites by Human Intestinal Acteria

目的 :对黄芩汤及其经肠道菌群作用后的代谢产物的体内保肝作用进行比较研究。方法 :使用普通和伪无菌小鼠 ,用D 半乳糖胺造成肝脏损伤模型 ,分别给予黄芩汤和黄芩汤的代谢产物 ,测定给药 3d后血清中丙氨酸氨基转换酶 (ALT)和天门冬氨酸氨基转换酶 (AST)的活性。结果 :普通小鼠口服黄芩汤后 ,高、中剂量组小鼠血清中ALT水平明显下降 ,与模型组产生显著差异 ;伪无菌小鼠口服黄芩汤代谢产物之后 ,高、中剂量组血清中ALT水平显著下降 ,但黄芩汤 3个剂量组对其无相似的药理作用。结论

Objective: To compare the protective activ it y of liver injury induced by D-galactosamine(GalN) between Huangqin-Tang a nd their metabolites by human intestinal bacteria(HIB). Method: The liver injuries in conventional and pseudo-germfree mice were induced by GalN. After oral administration of Huangqin-Tang and their metabolites mixtu res by HIB, the serum transaminase (ALT and AST) activities were detected. Result: In conventional mice, large and medium doses (20 and 10 g·kg -1 ) of Huangqin-Tang decoction significantly reduced the increase o f serum ALT activity after 18h GalN treatment. In pseudo-germfree mice, metabol ites significantly reduced the ALT levels. However, Huangqing-Tang didn't affec t the ALT levels in this kind of mice. To all of the animals, AST levels remaine d the same after oral Huangqin-tang or their metabolites. Conclusion : The metabolism by intestinal bacteria plays a role in pharmacologic al effects of constituents of Chinese herbal medicine. The metabolites of the co nstituents by intestinal bacteria were the real active components in vivo.