姜油体外抗氧化活性和对细胞DNA损伤防护作用的实验研究

Antioxidation Activity and Protective Effecton of Ginger oil on DNA Damage in Vitro

目的 :探讨姜油的体外抗氧化活性和对DNA损伤的防护作用。方法 :采用化学发光法测定姜油对氧自由基的清除能力 ;用比色法检测对人红细胞溶血和兔肝匀浆丙二醛 (MDA )生成的影响 ;采用单细胞凝胶电泳法检测对淋巴细胞DNA损伤的影响。结果 :与对照组比较 ,姜油可明显抑制本底的发光强度 ;对H2 O2 诱导的红细胞溶血和DNA损伤有明显的抑制作用 ;且能抑制兔肝匀浆MDA的生成 ,抑制率为 48.16%。结论 :姜油具有较强的氧自由基清除能力和防护DNA损伤的作用 ,是天然的抗氧化剂 。

Objective: To study the antioxidation ac tivity and protective effect of ginger oil on DNA damage. Method: Chemical lig ht assay was used to detect the oxygen radicals scavenging capacity of ginger oi l. The ery throcyte oxidation damage was induced by H 2O 2. The effect of ginger oil on o xidative erythrocyte was observed by the colorimetric analysis assay, and the content of malondialdehyde (MDA) in rabit hepatocyte was measured. The anaylsis of DNA damage was made with single cell gel electrophoresis(SCGE) techique. Result: Ginger oil might de crease light value compared with control group and inhibited erythrocyte ox idation damage. Compared with that in control group, the degress of DNA damage reduced significantly in the protected groups. Ginger oil might decrease the con tent of MDA remarkably and inhitibition rate was 48.16%. Conclusion: Ginger oil has dominantive protective effect on DNA damage induced by H 2O 2. Ginger oil might act as a scavenger of oxygen radical and might be us ed as an antioxidant.