精氨酸对四氧化二氮染毒鼠脏器功能的保护作用

Protective effect of arginine on the organ of mice which intoxicated with nitric tetroxide

目的 探讨精氨酸对液体火箭推进剂四氧化二氮 (N2 O4)中毒鼠肝、肾、心等脏器功能的保护作用 ,以寻找可以救治四氧化二氮中毒致伤的药物。方法 LACA雄性小鼠 ,18～ 2 2g ,共 30只。按体重随机分成 3组 :对照组、中毒未治组、精氨酸治疗组。模型制作 :经过预实验后 ,将小鼠放置在染毒柜中 ,使用微量注射器定量抽取液态N2 O4,注入 12 0L染毒柜中 ,维持染毒柜内 [N2 O4]=30× 10 -6g/m3 ,染毒时间 15min ,制备小鼠的吸入性中毒损伤模型。分组 :对照组不予处理 ;模型组同法给予腹腔注射生理盐水 0 .1mL/只 ,3次 /d ,共 3d ;治疗组于染毒后给予腹腔注射精氨酸注射液 0 .1mL/只 ,3次 /d ,共 3d。结果 染毒模型组小鼠的各项生化指标检测结果较对照组明显变化 ,治疗组小鼠的各项生化指标检测结果有较大程度的改善。结论 精氨酸可以保护N2 O4中毒致伤小鼠的脏器功能 ,对于救治N2 O4中毒损伤具有一定效果。

Objective To investigate the protective effect of arginine on the organ of the mice which intoxicated with nitric tetroxide, and then to look for new method to deal with intoxication of nitric tetroxide. Methods Thirty male LACA were divided randomly into 3 groups according to body weight: control group, intoxication group, arginine treatment group. To prepare the intoxication model:nitric tetroxide was given to mice by inhalation for 15 minutes,and keeping[N_2O_4]= 30 ppm. The control group was not treated; the intoxication group was only injected with 0.1 mL NS each mice;the arginine treatment group was injected with arginine (0.1 mL,3 times per day,3 days ).Results The mice′s hemanalysis of the intoxication group was worse than that of the control group, and the mice′s hemanalysis of the arginine treatment group was better than that of the intoxication group. Conclusion Treatment with arginine could protect the dysfunction of liver and kidney of the mice intoxicated with nitric tetroxide,and arginine could help to treat the injured mice of nitric tetroxide.