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bcl-2/bcl-xL反义寡核苷酸对乳腺癌细胞增殖和凋亡的影响 被引量：1

The Effects of bcl-2/bcl-xL Antisense Oligodeoxyneucleotides on Proliferation and Apoptosis of Breast Cancer Cell Line MCF-7
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摘要 目的　观察靶向bcl 2 /bcl xL基因的反义寡核苷酸 (ASODN )对乳腺癌细胞株MCF 7增殖和凋亡的影响。方法　将bcl 2 /bcl xLASODN及其对照序列通过脂质体转染MCF 7细胞。采用MTT法测定MCF 7细胞增殖 ,荧光显微镜定量检测MCF 7细胞凋亡率。结果　与对照序列相比 ,bcl 2 /bcl xLASODN能抑制MCF 7增殖和诱导其凋亡 (P <0 .0 1)。结论　bcl 2 /bcl xLASODN在乳腺癌治疗方面作为 Objective To observe the effect of antisense oligodexynucelotides(ASODN) targeting bcl-2/bcl-xL on cell proliferation and apoptosis of breast cancer cell line MCF-7.Methods bcl-2/bcl-xL ASODN and control sequence were transfected into MCF-7 with lipofectin.The MCF-7 cell growth was assessed by MTT and cell apoptosis was detected by quantitative fluorescence microscope.Results Compared with control sequence,bcl-2/bcl-xL ASODN could inhibit the growth of MCF-7 and induce apoptosis(P<0.01).Conclusion The bcl-2/bcl-xL-antisense oligonucleotide has a value to further study as a novel compound for breast cancer therapy.
作者 章骏 杨建辉
机构地区 上海市市东医院肿瘤外科
出处 《实用癌症杂志》 2003年第5期457-459,共3页 The Practical Journal of Cancer
关键词 BCL-2 BCL-X L反义寡核苷酸 乳腺癌 细胞增殖 细胞凋亡 Breast cancer bcl-2 gene bcl-xL gene Antisense oligodeoxyneucleotides(ASODN)
分类号 R737.9 [医药卫生—肿瘤]
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参考文献10

  • 1Green DR,Reed JC. Mitochondria and apoptosis[J]. Science, 1998,281(3 ) : 1309.
  • 2Holmqvist P, Lundstrom M, Stal O. Apoptosis and bcl-2 expression in relation to age, tumor characteristics and prognosis in breast cancer. South-East Sweden Breast Cancer Group[J]. Int J Biol Markers, 1999,14(7) :84.
  • 3Olopade OI, Adeyanju MO, Sara AR, et al. Overexpression of BCL-xprotein in primary breast cancer is associated with high tumor grade and nodal metastases[J]. Cancer J Sci Am, 1997,3(11) :230.
  • 4Zangemeister-Wittke U, Leech SH, Olie RA, et al. A novel bispeeific antisense oligonucleotide inhibiting both bcl-2 and bcl-xL expression efficiently induces apoptosis in tumor cells[J]. Clin Cancer Res,2000,6(6):2547.
  • 5Ziegler A, Luedke GH, Fabbro D, et al. Induction of apoptosis in small-cell lung cancer cells by an antisense oligodeoxynucleotide targeting the Bcl-2 coding sequence[J]. J Natl Cancer Inst, 1997,89 (14) : 1027.
  • 6Chi KC,Wallis AE, Lee CH,et al. Effects of bcl-2 modulation with G3139 antisense oligonucleotide on human[J]. Breast Cancer Res Treat,2000,63(3) :199.
  • 7Simoes-Wust AP,Olie RA,Gautschi O,et al. bcl-xl antisense treatment induces apoptosis in breast carcinoma cell[J ]. Int J Cancer,2000,87(4) :582.
  • 8Olie RA, Hafner C, Kuttel R, et al. bcl-2 and bcl-xL antisense oligonucleotides induce apoptosis in melanoma cells of different clinical stages[J]. J Invest Dermatol 2002,118(3):505.
  • 9Strasberg RM, Zangemeister-Wittke U, Rieber M. p53-Independent induction of apoptosis in human melanoma cells by a bcl-2/bcl-xL bispecific antisense oligonucleotide [J]. Clin Cancer Res, 2001,7(5) : 1446.
  • 10Gautschi O,Tschopp S,Olie RA,et al. Activity of a novel bcl-2/bcl-xL-bispecifie antisense oligonueleotide against tumors of diverse histologic origins [J]. J Natl Cancer Inst,2001,93(6) :463.

同被引文献17

  • 1马超,匡安仁,唐恭顺,黄蕤.^(125)I-寡核苷酸-长循环脂质体的制备[J].四川大学学报（医学版）,2005,36(3):415-418. 被引量：4
  • 2Fenske DB, MacLachlan I, Cullis PR. Long-circulating vectors for the systemic delivery of genes. Curr Opin Mol Ther,2001 ,3 (2) : 153-158.
  • 3Song LY, Ahkong QF, Rong Q, etal. Characterization of the inhibitory effect of PEG-lipid conjugates on the intracellular delivery of plasmid and antisense DNA mediated by cationic lipid liposomes. Biochim Biophys Acta, 2002 , 1558(1) : 1-13.
  • 4Moreno A, Figueras A, Lloveras B,et al. Apoptosis in ductal carcinoma in situ of the breast. Breast J, 2001 ; 7 (4) : 245-248.
  • 5Chi KC, Wallis AE, Lee CH, et al. Effects of Bcl-2 modulation with G3139 antisense oligonucleotide on human breast cancer cells are independent of inherent Bcl-2 protein expression. Breast Cancer Res Treat,2000,63(3):199-212.
  • 6Simoes-Wust AP, Olie RA, Gautschl O, et al. Bcl-xl antisense treatment induces apoptosis in breast carcinoma cells. Int J Cancer, 2000 ; 87 (4):582-590.
  • 7Akhtar S, Hughes MD, Khan A, et al. The delivery of antisense therapeutics. Adv Drug Deliv Rev,2000344(1) :3-21.
  • 8Juliano R, Alam MR, Dixit V, et al. Mechanisms and strategies for effective delivery of antisense and siRNA oligonucleotides. Nucleic Acids Res,2008,36(12):4158-4171.
  • 9Zelphati O, Szoka FC Jr. Intracellular distribution and mechanism of delivery of oligonucleotides mediated by cationic lipids. Pharm Res,1996,13(90) :1367-1372.
  • 10Gautschi O, Tschopp S, Olie RA, et al. Activity of a novel bcl-2/bcl-xL-bispecific antisense oligonucleotide against tumors of diverse histologic origins. J Natl Cancer Inst, 2001 , 93 (6) : 463-471.

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实用癌症杂志
2003年 第5期

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